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中国精品科技期刊2020
魏嘉,李丽,刘军,等. 混菌发酵对提高棉籽粕蛋白和降低游离棉酚的影响[J]. 食品工业科技,2024,45(16):231−238. doi: 10.13386/j.issn1002-0306.2023100091.
引用本文: 魏嘉,李丽,刘军,等. 混菌发酵对提高棉籽粕蛋白和降低游离棉酚的影响[J]. 食品工业科技,2024,45(16):231−238. doi: 10.13386/j.issn1002-0306.2023100091.
WEI Jia, LI Li, LIU Jun, et al. Effects of Mixed Bacteria Fermentation on Cottonseed Meal[J]. Science and Technology of Food Industry, 2024, 45(16): 231−238. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2023100091.
Citation: WEI Jia, LI Li, LIU Jun, et al. Effects of Mixed Bacteria Fermentation on Cottonseed Meal[J]. Science and Technology of Food Industry, 2024, 45(16): 231−238. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2023100091.

混菌发酵对提高棉籽粕蛋白和降低游离棉酚的影响

Effects of Mixed Bacteria Fermentation on Cottonseed Meal

  • 摘要: 为了降低棉籽粕中游离棉酚的含量,提高棉籽蛋白品质,本研究采用两阶段发酵法对棉籽粕进行处理,即先添加屎肠球菌(Enterococcus faecalis,E),再添加米曲霉(Aspergillus oryzae,M1)进行分段发酵,通过单因素实验和正交试验优化混菌发酵工艺条件。结果表明,当屎肠球菌接种量1.5%、发酵温度37 ℃、发酵时间24 h、米曲霉接种量1.5%、发酵温度30 ℃、发酵时间96 h、棉粕物料初始水分50%时,棉籽粕的粗蛋白含量从39.6%提高到49.64%,酸溶蛋白含量从4.79%提高到36.58%,游离棉酚的含量从1370 mg/kg降低至45.32 mg/kg,其降解率达到了96.7%,研究结果为棉粕的高值化利用提供了科学依据。

     

    Abstract: To reduce the content of free gossypol in cottonseed meal and improve the quality of cottonseed protein, this study used a two-stage fermentation method to treat cottonseed meal. Enterococcus faecalis (E) was first added, and then Aspergillus oryzae (M1) was added for staged fermentation. The fermentation conditions of mixed bacteria were optimized by single factor and orthogonal experiments. The results showed that when the inoculation amount of Enterococcus faecium was 1.5%, the fermentation temperature was 37 ℃, the fermentation time was 24 h, the inoculation amount of Aspergillus oryzae was 1.5%, the fermentation temperature was 30 ℃, the fermentation time 96 h, and the initial fermentation moisture of cottonseed meal was 50%, the crude protein content of cottonseed meal increased from 39.6% to 49.64%, the acid-soluble protein content increased from 4.79% to 36.58%, and the free gossypol content decreased from 1370 mg /kg to 45.32 mg/kg, and the detoxification rate reached 96.7%. The results provide a scientific basis for the high-value utilization of cottonseed meal.

     

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