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中国精品科技期刊2020
黄后培,袁媛,汪卓,等. 罗非鱼头硫酸软骨素对RAW 264.7细胞和小鼠免疫刺激活性研究[J]. 食品工业科技,xxxx,x(x):1−8. doi: 10.13386/j.issn1002-0306.2023120214.
引用本文: 黄后培,袁媛,汪卓,等. 罗非鱼头硫酸软骨素对RAW 264.7细胞和小鼠免疫刺激活性研究[J]. 食品工业科技,xxxx,x(x):1−8. doi: 10.13386/j.issn1002-0306.2023120214.
HUANG Houpei, YUAN Yuan, WANG Zhuo, et al. Immunostimulatory Activity of Tilapia-Head Chondroitin Sulfate in RAW 264.7 Cells and Mice[J]. Science and Technology of Food Industry, xxxx, x(x): 1−8. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2023120214.
Citation: HUANG Houpei, YUAN Yuan, WANG Zhuo, et al. Immunostimulatory Activity of Tilapia-Head Chondroitin Sulfate in RAW 264.7 Cells and Mice[J]. Science and Technology of Food Industry, xxxx, x(x): 1−8. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2023120214.

罗非鱼头硫酸软骨素对RAW 264.7细胞和小鼠免疫刺激活性研究

Immunostimulatory Activity of Tilapia-Head Chondroitin Sulfate in RAW 264.7 Cells and Mice

  • 摘要: 本文采用了RAW 264.7细胞和免疫抑制模型,通过体外、体内实验研究罗非鱼头硫酸软骨素(CS)的免疫刺激活性。结果表明,罗非鱼头硫酸软骨素(CS)可以诱导RAW 264.7细胞产生NO、IL-1β、IL-6、IL-10、TNF-α;环磷酰胺(CTX)诱导小鼠的胸腺萎缩(胸腺指数(0.11±0.01)%),低剂量CS(胸腺指数(0.17±0.03)%)、高剂量CS(胸腺指数(0.18±0.02)%)干预后,显著上调了小鼠胸腺指数(P<0.05);同时,CS干预显著上调CTX诱导小鼠脾脏指数(P<0.05),上调绒毛长度和V/C值并且高剂量CS显著上调了绒毛长度(330.92±21.55)μm(P<0.05);另外,CS对CTX诱导小鼠血清sIgA的分泌有促进作用。这些结果表明CS可以改善免疫抑制小鼠肠道屏障损伤及免疫功能。因此,本研究可为罗非鱼硫酸软骨素的肠道黏膜免疫调节作用提供理论基础,并表明罗非鱼头硫酸软骨素(CS)是一种潜在的可替代鲨鱼硫酸软骨素的免疫调节多糖,并可能成为干预免疫疾病相关功能食品的潜在材料。

     

    Abstract: In this study, RAW 264.7 cells and immunosuppressive models were used to investigate the immunostimulatory activity of Tilapia-head chondroitin sulfate (CS) through in vitro and in vivo experiments. The results showed that Tilapia-head chondroitin sulfate (CS) could induce the produce of NO, IL-1β, IL-6, IL-10 and TNF-α in RAW 264.7 cells. Cyclophosphamide (CTX) induced thymic atrophy in mice (thymic index (0.11±0.01)%), while thymic index was significantly up-regulated by low-dose CS (0.17±0.03)% and high-dose CS (0.18±0.02)% interventions (P<0.05). Meanwhile, CS significantly increased spleen index in CTX-induced mice (P<0.05), promoted villus length and V/C value, with high-dose CS significantly increased villus length (330.92±21.55) μm (P<0.05). Furthermore, CS enhanced the secretion of serum sIgA in CTX-induced mice, indicating that CS could improve intestinal barrier damage and immune function in immunosuppressive mice. Therefore, this study provides a theoretical basis for the intestinal mucosal immune regulation of Tilapia chondroitin sulfate, and suggests that Tilapia-head chondroitin sulfate (CS) is a potential immunoregulatory polysaccharide that could replace shark chondroitin sulfate, potentially serving as a promising material for functional foods aimed at intervening in immune-related diseases.

     

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