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中国精品科技期刊2020
林烨欣,阎光宇,王雅茹,等. 蛹虫草可溶性β-葡聚糖的分离纯化、结构表征及降血糖活性[J]. 食品工业科技,2025,46(2):1−9. doi: 10.13386/j.issn1002-0306.2024020067.
引用本文: 林烨欣,阎光宇,王雅茹,等. 蛹虫草可溶性β-葡聚糖的分离纯化、结构表征及降血糖活性[J]. 食品工业科技,2025,46(2):1−9. doi: 10.13386/j.issn1002-0306.2024020067.
LIN Yexin, YAN Guangyu, WANG Yaru, et al. Isolation and Purification, Structural Characterization, Hypoglycemic Activity of Soluble β-glucan from Cordyceps chrysanthemi[J]. Science and Technology of Food Industry, 2025, 46(2): 1−9. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2024020067.
Citation: LIN Yexin, YAN Guangyu, WANG Yaru, et al. Isolation and Purification, Structural Characterization, Hypoglycemic Activity of Soluble β-glucan from Cordyceps chrysanthemi[J]. Science and Technology of Food Industry, 2025, 46(2): 1−9. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2024020067.

蛹虫草可溶性β-葡聚糖的分离纯化、结构表征及降血糖活性

Isolation and Purification, Structural Characterization, Hypoglycemic Activity of Soluble β-glucan from Cordyceps chrysanthemi

  • 摘要: 菌菇来源可溶性β-葡聚糖具有良好的生物活性,为开发利用蛹虫草中的β-葡聚糖(CMBG),本研究以蛹虫草子实体为原料,采用蛋白酶酶解、大孔树脂吸附、DEAE离子交换柱层析进行纯化,以脱色率及β-葡聚糖保留率进行加权评分,摸索建立最佳纯化工艺条件。对获得的β-葡聚糖进行结构分析,并研究其体外降血糖活性。结果显示:大孔树脂HPD-750具有最佳的吸附效果,其吸附最优条件为:上样流速5 mL/min、上样浓度4 mg/mL、上样体积400 mL、树脂质量40 g。利用DEAE-FF纤维素交换柱分离出两个β-葡聚糖组分CMBG-1和CMBG-2,相对分子量分别为13.57×104 Da和14.36×104 Da;两者均由Gal,Glc,Ara三种单糖组成,其摩尔比分别为1.28:5.27:1.01和0.71:4.43:0.80。通过刚果红分析得出CMBG-1具有三螺旋结构,而CMBG-2不具备三螺旋结构,二者对α-淀粉酶及α-葡萄糖苷酶抑制率都与样品的浓度呈正相关,其对α-淀粉酶抑制的IC50分别为2.68和4.32 mg/mL,对α-葡萄糖苷酶抑制的IC50分别为2.37和3.01 mg/mL,拥有三螺旋结构的CMBG-1的降血糖活性明显高于不具备三螺旋结构的CMBG-2,在功能食品开发方面具有良好的前景。

     

    Abstract: Soluble β-glucan from mushroom source had good biological activity, in order to develop and utilize β-glucan (CMBG) in Cordyceps chrysanthemi, this study used Cordyceps chrysanthemi substrate as raw material, and was purified by proteolytic enzyme digestion, adsorption of macroporous resin, and chromatography of DEAE ion-exchange columns, and the decolorization and β-glucan retention were weighted and scored to feel out the establishment of the optimal purification process conditions. The obtained β-glucan was structurally analyzed and its in vitro hypoglycemic activity was investigated. The results showed that the macroporous resin HPD-750 had the best adsorption effect, and the optimal conditions for adsorption were as follows: up-sample flow rate of 5 mL/min, up-sample concentration of 4 mg/mL, up-sample volume of 400 mL, and mass of the resin of 40 g. Two β-glucan fractions, CMBG-1 and CMBG-2, with relative molecular weights of 13.57×104 Da and 14.36×104 Da, respectively, were isolated using a DEAE-FF cellulose exchange column. Both consisted of three monosaccharides, Gal, Glc, and Ara, with molar ratios of 1.28:5.27:1.01 and 0.71:4.43:0.80, respectively. The Congo red analysis showed that CMBG-1 had a triple-helix structure, while CMBG-2 had no triple-helix structure, and the inhibition rates of both of them on α-amylase and α-glucosidase were positively correlated with the concentrations of the samples, and the IC50 of their inhibition on α-amylase were 2.68 and 4.32 mg/mL, and those on α-glucosidase were 2.37 and 3.01 mg/mL, respectively. The hypoglycemic activity of CMBG-1, which possessed a triple-helix structure, was significantly higher than that of CMBG-2, which did not possess a triple-helix structure, and it had good prospects for the development of functional foods.

     

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