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中国精品科技期刊2020
林育钊,彭泽甜,陈洪彬,等. ε-聚赖氨酸对西番莲果腐病菌可可毛色二孢的抑制作用及其机理[J]. 食品工业科技,2024,45(16):191−200. doi: 10.13386/j.issn1002-0306.2024040047.
引用本文: 林育钊,彭泽甜,陈洪彬,等. ε-聚赖氨酸对西番莲果腐病菌可可毛色二孢的抑制作用及其机理[J]. 食品工业科技,2024,45(16):191−200. doi: 10.13386/j.issn1002-0306.2024040047.
LIN Yuzhao, PENG Zetian, CHEN Hongbin, et al. Antifungal Effects of ε-Poly-l-lysine on Lasiodiplodia theobromae Causing Fruit Rot of Passiflora caerulea L. and Its Mechanism[J]. Science and Technology of Food Industry, 2024, 45(16): 191−200. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2024040047.
Citation: LIN Yuzhao, PENG Zetian, CHEN Hongbin, et al. Antifungal Effects of ε-Poly-l-lysine on Lasiodiplodia theobromae Causing Fruit Rot of Passiflora caerulea L. and Its Mechanism[J]. Science and Technology of Food Industry, 2024, 45(16): 191−200. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2024040047.

ε-聚赖氨酸对西番莲果腐病菌可可毛色二孢的抑制作用及其机理

Antifungal Effects of ε-Poly-l-lysine on Lasiodiplodia theobromae Causing Fruit Rot of Passiflora caerulea L. and Its Mechanism

  • 摘要: 为研究ε-聚赖氨酸(ε-poly-l-lysine,ε-PL)对西番莲果实采后果腐病菌可可毛色二孢(Lasiodiplodia theobromae)的抑制作用和抑菌机理,本实验测定了ε-PL对L. theobromae菌丝生长、电导率、丙二醛含量、细胞内物质渗漏、细胞膜和细胞壁组分含量、腺苷三磷酸酶活性、碘化丙啶染色和傅里叶变换红外光谱变化,及对L. theobromae侵染所致西番莲采后果腐病的抑制效果。体外实验结果表明,ε-PL对L. theobromae菌丝生长具有抑制作用,且随着ε-PL浓度升高而增强,10 mg/mL为最佳抑制浓度;同时显微镜结果表明ε-PL可改变L. theobromae菌丝形态,使其卷曲和弯折。另外,ε-PL提高了L. theobromae菌丝体的电导率和丙二醛含量,促进核酸和蛋白质等细胞内物质渗漏,降低麦角固醇、总脂质和几丁质含量及腺苷三磷酸酶活性,减少亚甲基、甲基、羟基、碳氧键等数量。此外,ε-PL延缓了接种L. theobromae西番莲果实采后果腐病发生。因此,ε-PL可破坏L. theobromae细胞膜与细胞壁结构,促进内容物溶出,扰乱能量代谢,破坏生物大分子结构,进而起到抑菌作用。

     

    Abstract: To investigate the inhibitory effects and mechanism of ε-poly-l-lysine (ε-PL) against the Lasiodiplodia theobromae causing fruit rot on postharvest passions, the changes of L. theobromae mycelial growth, conductivity, malonaldehyde content, intracellular materials leakage, contents of cell membrane and cell wall components, adenosine triphosphatase activity, propidium iodide staining, and Fourier transform infrared spectroscopy were determined after being treated with ε-PL, and the inhibition effects of ε-PL on L. theobromae-induced fruit rot of harvested passion fruits were studied. In vitro assay showed that ε-PL inhibited the mycelial growth of L. theobromae, and the inhibitory effects increased with increasing ε-PL concentration. The optimal inhibitory concentration of ε-PL was 10 mg/mL. ε-PL also changed the mycelial morphology of L. theobromae, resulting in the mycelial distortion. Furthermore, ε-PL might increase the conductivity and malondialdehyde content, promote the leakage of intercellular nucleic acid and protein, decrease the contents of ergosterol, total lipids and chitin, reduce the activity of adenosine triphosphate, and lower the number of methylene, methyl, hydroxyl and carbon-oxygen bonds in mycelium of L. theobromae. Furthermore, ε-PL could suppress the L. theobromae-induced fruit rot development of harvested passion fruits. Therefore, ε-PL could display the antifungal activity on L. theobromae through destroying the structure of cell membrane and cell wall, promoting the leakage of intracellular materials, disordering the energy metabolism, and destroying the structure of biological macromolecules.

     

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