Abstract: According to special quality requirements of barley grass health products to barley varieties, the chlorophyll (SPAD value) , soluble solids content, betain and flavonoid of the 100 selected cultivars were determined.The results showed that four contents displayed a wide range in 100 barley cultivars, and the contents of chlorophyll (SPAD value) , soluble solids, betain and flavonoid were 28.63 ～ 56.10, 36.92 ～ 110.26mg/g fresh weigh (FW) , 296.33 ～ 8149.00μg/g FW and 304.42 ～ 7308.60μg/g FW, respectively.The correlation analysis indicated that the soluble solids content was positively correlated to betain and flavonoid, and the SPAD value was correlated with betain.Based on comprehensive evaluation of bioactive substances, 10 barley varieties fitted for producting barley grass health products were selected in this study.