苦白蹄乙醇提取物体外抗肿瘤活性研究
Anti-tumor effect of ethanol extracts from Fomitopsis officinalis (Vill.:Fr.) Bond.in vitro
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摘要: 探讨苦白蹄乙醇提取物在体外的抗肿瘤活性。采用磺酰罗丹明染色法（SRB法）考察苦白蹄乙醇提取物对人肝癌细胞BEL-7404、人肺腺癌细胞H-1299、人胃腺癌细胞SGC-79013种人恶性肿瘤细胞的体外抗肿瘤活性。结果显示,苦白蹄乙醇提取物对BEL-7404、SGC-7901两株肿瘤细胞具有比较明显的抑制效果,其半数抑制浓度（IC50）分别为73.5、80μg·mL-1,且抑制率随药物浓度的升高而增大,呈剂量效应关系,但对H-1299的抑制率相对较低。细胞周期实验显示,BEL-7404细胞在苦白蹄乙醇提取物的作用下,G2/M期的细胞比例呈下降趋势,S期细胞比例增大,细胞周期阻滞于S期。苦白蹄乙醇提取物具有比较明显的体外抗肿瘤活性。Abstract: The anti-tumor effect of ethanol extracts from Fomitopsis officinalis (Vill.:Fr.) Bond.on various human tumor cell lines in vitro was evaluated.The sulforhodamine B (SRB) assay was employed to evaluate the anti-tumor effect of ethanol extracts from Fomitopsis officinalis (Vill.:Fr.) Bond.on BEL-7404, H-1299 and SGC-7901 cells in vitro.The results showed that ethanol extracts displayed obvious inhibitory effect on the proliferation of BEL-7404 and SGC-7901 in a dose-dependent manner, while their inhibitory effect on H-1299 was not evident.The IC50 value of BEL-7404 and SGC-7901 were 73.5μg·mL-1 and 80μg·mL-1 respectively.The cell cycle of BEL-7404 treated by ethanol extracts changed.The ratio of cultured cell declined in G2/M phase, while the cell in S phase increased.The cell cycle arrested in S phase.Ethanol extracts from Fomitopsis officinalis (Vill.:Fr.) Bond.had relatively obvious anti-tumor effect in vitro.
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