荚果蕨中多糖的超声辅助提取及其DPPH自由基清除作用的研究
Ultrasonic extraction of polysaccharides from Matteuccia struthiopteris and their DPPH free radical scavenging activity
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摘要: 以荚果蕨（Matteuccia struthiopteris（L.）Todaro）根状茎为材料,采用单因素实验和正交实验探讨超声辅助提取多糖的最佳工艺条件,应用1,1-二苯基苦基苯肼（DPPH）自由基清除法评价荚果蕨根状茎中多糖的抗氧化活性。实验结果表明,超声辅助提取该多糖的最佳工艺参数为:提取温度为80℃、料水比为1:50（g/mL）、超声时间为30min、超声功率为150W。在此条件下提取的荚果蕨根状茎、营养叶、孢子叶3个部位中粗多糖的提取率分别为9.67%、3.30%、3.43%。同时,荚果蕨根状茎中多糖有较强的抗氧化作用,对DPPH自由基半数抑制浓度（IC50）为68.68μg/mL。研究荚果蕨多糖的超声辅助提取工艺及抗氧化活性,对荚果蕨天然保健品的开发利用有重大指导意义。Abstract: The condition for ultrasonic extraction of polysaccharides from Matteuccia struthiopteris were optimized by one-factor-at-a-time and orthogonal array design methods.Polysaccharide from Matteuccia struthiopteris was tested with DPPH· to estimate their antioxidative activity.The results showed that the best extraction conditions were determined as below:ethanol liquid ratio was 1:50 (g/mL) , extraction temperature was 80℃, ultrasonic power was 150W, ultrasonic time was 30min.The extraction yield of polysaccharide in rhizome, trophophyll and sporophyl was 9.67%, 3.30% and 3.43% respectively.The polysaccharide had high antioxidative activity, revealing an IC50 of 68.68μg/mL.Investigating ultrasonic traction of polysaccharides from matteuccia struthiopteris and their DPPH free radical scavenging activity, it is a great direction significance in the development and utilization of matteuccia struthiopteris.
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