Abstract: The solid phase extraction followed by high performance liquid chromatography-fluorescence detection of six bisphenols was established and applied to investigated the levels of bisphenols in 31 canned beers purchased from Chinese markets.The method detection limits were 0.02～0.09ug/L and the method detection ranges were 0.02～50.00ug/L with correlation coefficients higher than 0.99.The recovery and the relative standard deviation were 80.67%～101% and 1.01%～4.84%, respectively.Expect Bisphenol E (BPE) , Bisphenol A (BPA) , Bisphenol A diglycidyl ether (BADGE) , Bisphenol F diglycidyl ether (BFDGE) , Bisphenol B (BPB) and Bisphenol F (BPF) were detected in samples with average concentrations of 2.99, 0.41, 0.18, 0.12, 0.12ug/L, seperately.And their corresponding detection rates were 100%, 83.9%, 6.4%, 12.9% and 6.4%, respectively.The result suggested that BPA and BFDGE were the major bisphenols present in canned beers and their mean dietary intakes were estimated at 0.0160, 0.0022ug/kg of body weight/day which was below the regulation of European Community (EC) on 50ug/kg BPA of body weight/day.The levels of BPA and BADGE did not exceed the regulations of EC.However, the presence of BFDGE was forbidden.In addition, there are no regulations on BPB, BPE or BPF.