Abstract: Bacteriocins can be against numerous foodborne pathogen and spoilage organisms, hence researchers at home and aboard are interested in bacteriocins, especially, bacteriocin from lactic acid bacteria (LAB) are focused most.However, for bacteriocin could be applied to biotechnological fields better, it is essential to illuminate its biochemical structure and its mode of action, which needs that bacteriocins is purified to homogeneity.The main technologies used for the purification and detection of numerous bacteriocins was described, the exploration of bacteriocins potential in LAB was also discussed.