Abstract: By using the method of tissue culture endogenous mould from Xinjiang quality wine grape“Chi xiazhu” were separated.24 kinds of endogenous moulds were getted. From the time dimension, there were 11 isolates in autumn, 8 isolates in spring and 5 isolates in summer. From the distribution of tissue, there were 7 isolates from roots, 5 isolates in plant stem, 9 isolates from leaves and 3 isolates from fruit of wine grape. By morphological identification C-2-J-6 and C-1-G-3 were found with high ability of producing polyphenols, which belonged to the genus syncephalastrum and Penicillium.