Abstract: According to the exogenous insert sequences of the transgenic maize event Bt176 connected with the maize genome sequences, the event specific primers were designed and screened as well as the reaction system and the parameters were optimized. An event specific loop-mediated isothermal amplification method for the detection of transgenic maize event Bt176 was established in this paper.Moreover, an evaluation of this method on its specificity, sensitivity and stability was performed.The evaluation results showed the LOD of 0.5% , and both the specificity and stability of 100%.