Abstract: The ultrasonic-enzymatic extraction process was used to extract craft of polyphenols in Korean pine bark. The total polyphenol yield, DPPH radical scavening was used to indicate. The optimum conditions was obtained through orthogonal test based on single factor experiment.The results showed that the optimal ultrasonic- enzymatic extraction condition was as follows:ultrasonic temperature 60℃, ultrasonic time 80min, ultrasonic power 250W, enzyme solution temperature 45℃, adding amount of enzyme 4.0% , enzyme solution time 70min and pH4.8. The polyphenol extraction rate was 78.79mg /g and the DPPH radical scavening ratio was 63.68% .The method of the orthogonal test used to optimize the extraction craft of the ultrasonic-enzymatic extraction of the polyphenols in Korean pine bark was available. Under the optimum condition, the polyphenols in Korean pine bark had higher antioxidant activity.