Abstract: Exposed the carp to the solution at a mass concentration of Cd2+ (10mg/L) in advance to induce the synthesis of cadmium-metallothionein (Cd-MT) . Used atomic absorption method to measure the extraction amount of cadmium, and used Ellman’s reagent to measure the amount of sulfydryl content, and then set the two indicators as evaluation indexes. Synthesized traditional and classical methods to induce the extraction of batches of MT from different tissue of the carp. Then contrasted the extracted MT with the MT from different species of fish. Compared the MT content of different tissue, and used enzyme-linked immunoassay (ELISA) and hemoglobin/cadmium saturation method to measure the MT content. Finally there was a research of the properties of the MT from the carp. The results showed that Cd2+ (10mg/L) could induce the production of large amounts of MT, and the method could induce the synthesis of MT in bulk. The kinds and quantity of amino acid were basically identical to the standard MT of the fish. The measuring result of cysteine was same to the result of sulfydryl, both were free of aromatic amino. The properties of the MT were similar to those of mammal’s MT. The measuring results of the MT content of the carp’s liver and kidney were about the same by using ELISA and hemoglobin/cadmium saturation method.