Abstract: Metallothionein ( MT) could effectively represent the influences heavy metal in water has on creatures, so used MT as the biomarker and ELISA to measure the MT content in the tissue of carp.Then evaluated accumulation law in different tissue under the stress of heavy metal by the dynamic changes of MT content in carp tissues, and discussed the correlation between the MT in carp and heavy metal in water and carp tissues. The study was of significance for further research about the carp ’s accumulation and removal mechanism of heavy metal, its tolerance of different kinds of heavy metal, and the ascertainment of pollution source and level.