Abstract: Using 5th and 3th instar larvae of Dendrolimu kikuchii Matsumura as the tested strains, Oxford cup method was used to measure the inhibition effect of the 5 different extract from Ebenaceae Diospyroskaki leaf.The results showed that except petroleum ether of Ebenaceae Diospyroskaki leaf extract the other 4 different extract from extract had inhibition effect on Dendrolimu kikuchii Matsumura, and with the decreasing concentrations of its inhibition also reduced.Inhibition of order was Ethyl acetate extract>Chloroform extract>Ethanol extract>N-butyl alcohol extract, the four Ebenaceae Diospyroskaki leaf extract had a strong inhibition effect on 302 strains.Ebenaceae Diospyroskaki leaf extract could be used as the good new plant source biological pesticide to development and utilization.