Abstract: The relationship between Glycine max var sprout protective enzymes and functional components during germination time was studied.For the further study of germination Glycine max physiology of development status and nutritional change and provide a theoretical basis for reasonable utilization.The different periods of germination of black Glycine max, detection of various enzyme specific activity and functional components.Test results showed that the content of soluble protein in seeds was the minimum, during germination, 60h reached the highest 134.00mg/g dry weight (DW) ;Soluble polysaccharide in 12h reached the maximum at 77.47mg/g DW, then decreased gradually;Polyphenol content during the germination of 12h, 60h appeared two peaks, reached a peak of 1.91 mg/g DW in 60h.The specific activities of CAT in 12h content was the highest for 35.56 U/g protein (Pr) ;the 24h specific activities of SOD 299.33U/g Pr was the strongest, followed vigor weakened;POD enzyme activity in the germination of 12h reached the maximum of 67.98U/g Pr, and activity decreased followed;The PPO activity in cultured 12h achieved 64.09U/g Pr and it was the strongest vitality, then vigor reduced;The results showed that Glycine max during germination occurs over time corresponding to the physiological and biochemical changes to adapt to the growth and development of organisms.