Abstract: Objective:Research the inhibition of anthocyanins extracted from three kinds of Padus Mill fruits on the proliferation of HepG2 cell.Methods:The anthocyanins of different mass concentration extracted from three kinds of Padus effect on HepG2 cell for 24, 48 and 72h, then by placental blue staining method to calculate the number of active cells, and draw the proliferation curves.Test the inhibition rate of anthocyanins on the proliferation of cell by the method of MTT.Result:The anthocyanins of Padus virginiana increased the cell proliferation at the mass concentration of 0.05mg/mL.The anthocyanins of Padus racemosa and Prunus maackii caused the inhibition of cell proliferation in the selected concentration range, and the inhibition increased as the mass and time increased.After 48 hours, the number of IC50of the HepG2 cell to the anthocyanins was Padus Virginiana:1.07mg/mL, Prunus maackii:0.82mg/mL, Padus racemosa:0.64mg/mL, the order of inhibition was Padus racemosa>Prunus maackii>Padus Virginiana.Conclusion:The anthocyanins of three kinds of Padus Mill fruits caused inhibition on the proliferation of HepG2 cell effectively.