Abstract: Ca2+-ATPase activity as the indicator to reflect the relativity among propylene glycol, glycerol and sodium chloride. Response surface methodology was used to optimize the pretreatment conditions with the result:propylene glycol 2%+glycerol 3%+NaCl 1%. The results showed that pretreatment improved the remains of Ca2+-ATPase activity and salt-solubility of myofibrillar protein obviously. And the tissue structure was improved obviously. Drying temperature could influence protein denaturation obviously. The degeneration was more serious and the organization structure was damaged more seriously under the higher temperature.