Abstract: Leaves of coppice, leaves and leaves residual of high-forest tree of Eucommia ulmoides Olive were used to replace 40% cotton seed hull to cultivate pleurotus, and its effects on the process of mycelial growth and contents of protein, fat, related trace element of pleurotus were studied. Meanwhile, ultraviolet and visible spectrophotometer was used to measure the content of total flavonoid, and fluorescence spectroscopy was used to measure the content of selenium of pleurotus.Results showed that the process of mycelial growth were the best in the group of leaves residual of high-forest tree, and the contents of protein, fat, copper and manganese were the highest, which were 1.13, 1.73, 3.75 and 2.71 times as much as the blank group, respectively.In the group of leaves of high-forest tree, the contents of total flavonoid, calcium, sodium, potassium and magnesium were the highest, which were 1.49, 2.20, 7.20, 1.08 and 1.03 times same with the blank group, respectively. In the group of leaves of coppice, the contents of iron, zinc and selenium were the highest, which were 1.72, 2.10 and 2.38 times the same as the blank group, respectively. In addition, the content of selenium of pleurotus was 0.07524μg.g- 1in the group of leaves of coppice, which reached the standard of selenium enrichment.