Abstract: The dielectric constant and dielectric loss factor of scallop adductors were measured by the RF network analyzer over the frequency range of 0.3³GHz from the temperature 20℃ to 100℃.The effect of temperature and frequency on dielectric properties during microwave heating was found. The results showed that the dielectric constant and dielectric loss factor decreased with the growing of frequency at first at the same temperature, then they didn't change significantly.At frequency of 915MHz and 2450MHz, there was a negative correlation between the dielectric constant and temperature while the dielectric loss factor was positively correlated to temperature.In addition, the penetration depth decreased as temperature was growing.Study on the dielectric properties of scallop adductors provided theoretical basis for microwave sterilization.