Abstract: In order to study the spoilage characteristics of dominant spoilage bacteria, Pseudomonas koreensis, Clostridium fusiformis and Brochothrix thermosphacta were respectively inoculated into sterilized pork at the storage temperature of 4℃.Volatile compounds of pork with different storage time were analyzed by headspace solid micro extraction (HS-SPME) . Pork with storage time of 48, 96, 144h and dominant spoilage bacteria had a significant difference in volatile composition. As the time of cold-storage went on, the content of alkanes rose markedly, pork inoculated with Clostridium fusiformis and Brochothrix thermosphacta provided significantly higher levels of 3-hydroxy-2-Butanone and 3-methyl-Butanol, respectively. Contents of sulfhydryl methane and 3-sulfhydryl propylene were actually low. These results showed that at 0.05 significance level, there was significant difference in total volatile substance between pork inoculated by bacteria and control sample.