大孔树脂纯化血管紧张素酶抑制肽VLPVPR的工艺优化

Optimization of purification process of angiotensin converting enzyme inhibitor peptide VLPVPR with macroporous resin

摘要: 考察大孔树脂(AB8,D101,DM130,HPD100B,HPD826)对血管紧张素酶抑制肽VLPVPR的吸附性能及纯化效果,确立纯化VLPVPR的较优工艺。结果表明,HPD100B最适于VLPVPR的纯化。最优纯化工艺为:温度20℃,pH9.8,上样流速为3mL/(cm2·min),上样量达到180mL,洗脱液乙醇溶液浓度为60%,洗脱流速为0.25mL/(cm2·min),洗脱体积为45mL。此条件下,VLPVPR的解吸率达93.1%,纯度为28.8%,血管紧张素酶抑制率IC50为4.1μmol/L。

Abstract: To separate and purify angiotensin converting enzyme inhibitor peptide VLPVPR, the absorption capability and purification effect of VLPVPR on macroporous resin were evaluated. Adsorption and desorption experiments were carried out to screen the suitable macroporous resin for VLPVPR. HPD100B was the best macroporous resin for purification of VLPVPR. The optimization condition were temperature 20℃, pH9.8, feeding rate 3mL/ (cm2·min) , feeding volume 180mL, ethanol concertration 60%, elute rate 0.25mL/ (cm2·min) and elute volume 45mL. The desorption ratio was 93.1%, the purity ratio was 28.8% and the inhibition ratio (IC50) of the ACE was 4.1μmol/L.