餐饮废水中耐盐且具有蛋白降解能力菌株的分离鉴定及其生长特性的研究
Identification and study on growth characteristics of halophilous protein degradation bacterium in restaurant wastewater
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摘要: 通过耐盐试验和发酵试验,从餐饮废水中分离得到一株耐盐且高效降解蛋白的细菌(K-1),研究了盐浓度、pH及盐种类对其生长的影响。通过形态学、生理生化特征以及16SrDNA鉴定,初步鉴定该菌为不动杆菌(Acinetobacter),在20℃、2%盐浓度条件下蛋白质降解率可达68.82%。该菌株能够在NaCl浓度为10%的培养基中生长,最适生长pH为6.5,培养基中的Mg2+和K+对菌株生长既不促进也不抑制,Ca2+会抑制菌株的生长。Abstract: A halophilous protein degradation bacterium K- 1 was isolated from restaurant wastewater by salt tolerance test and fermentation test.The effect on K-1 growth of salt ratio, pH and salt species were investigated.Results showed that: Based on morphologic observation, biochemical characteristic and identification of 16SrDNA, K-1 belonged to the genus of Acinetobacter, which showed a ratio of protein degradation as high as 68.82% at20℃ with 2% salt.The strain could grow even with 10% NaCl's addition, and the optimum pH for bacteria growth was 6.5. The medium of Mg2 +and K+were neither promoting nor inhibiting the growth of K-1, Ca2 +could inhibit the growth of strain.