利用非培养技术初步研究古井贡酒窖泥细菌群落结构

Preliminary analysis on bacterial communities in GuJingGong pit mud through culture-free approach

摘要: 采用构建16S rDNA克隆文库的非培养方法,对古井贡酒窖泥细菌的群落结构及其多样性进行研究。结果表明:文库含有57个分类操作单元(OTU),793个克隆中未知菌类占60.78%,其中5个克隆得不到任何比对信息,占0.63%。已知菌类分别属于Firmicutes(31.16%)、Proteobacteria(3.55%)、Bacteroidetes(3.79%)、Synergistetes(0.63%)、Actinobacteria的Atopobium(0.13%)。已知菌类也多半为非培养细菌,其中Firmicutes占多数,但是其中也有一半的细菌相似度偏低,需要进一步验证。

Abstract: 16S rDNA clone library technique was conducted to analyze the bacterial community diversity in pit mud of GuJingGong liquor. All clone strains were classified to 56 OTUs. Of all the 793 clones, 60.78% were unknown species, and 5 sequences ( 0.63%) could not be found any information in Genebank.The known bacteria belonged to Firmicutes ( 31.16%) , Proteobacteria ( 3.55%) , Bacteroidetes ( 3.79%) , Synergistetes ( 0.63%) and Actinobacteria ( 0.13%) .Most of the known bacteria were also found as uncultured, of which, Firmicutes was the majority.Half of Firmicutes bacteria require further verification since they had a relatively low similarity in BLAST analysis.