Abstract: To investigate effects of high levels of DHA and EPA triglyceride on blood lipid and protection of the liver in hyperlipidemia mice.Male C57BL /6J mice were randomly divided into eight groups. The hyperlipidemia model mice was induced by feeding a diet containing high fat.After feeding high levels of DHA and EPA triglyceride for 15weeks, the indexes such as the contents of serum TC, TG, HDL-C and LDL-C, the contents of hepatic TC, TG and fatty acid were examined.Results showed that high levels of DHA and EPA triglyceride obviously decreased the contents of serum TC, TG and LDL- C, rose HDL- C, significantly caused a reduction of TC ( p < 0.01) , TG ( p <0.01) levels and an improvement of fatty acid forms in liver. The high levels of DHA and EPA triglyceride could improve the disturbance of blood lipid and liver lipid on hyperlipidemia mice.