Abstract: A method for the purification of total flavonoids from Sedum aizoon with AB-8 types of macroreticular adsorbents was studied by using combining static adsorption with elution and dynamic adsorption with elution.The adsorption and desorption rate of AB- 8 types of macroreticular adsorbents were calculated to determine the effectiveness, and influence factors of separation and purification of total flavonoids with the effects of pH value, elution ethanol concentration, elution time, the adsorption current velocity, the desorption current velocity, the eluant volume, the distilled water of elution valume. Its optimum separation and purification process conditions were determined as follows: the pH value of extracts was 5.0, 80% alcohol as the eluent, 24 h as eution time, 80 mL volume as the eluant volume and 100 mL volume as the distilled water of elution, the adsorption current velocity as 1~2mL/min and the desorption current velocity as 2mL /min.Under the conditions, the static adsorption capacity of AB-8 type of macroteticular adsorbent was 11 mg /g and the static adsorption rate was 68.3% while the desorption rate was94.5%.