响应面法优化复合酶提取香椿叶总膳食纤维的工艺参数

Optimization of process parameters for total dietary fiber from leaves of Toona Sinensis with compound enzyme method by response surface methodology

摘要: 以香椿叶为试材,采用单因素实验和响应面分析法对复合酶(纤维素酶∶淀粉酶=1∶1)提取香椿叶总膳食纤维(TDF)的工艺参数进行了优化。结果表明:在复合酶添加量0.5%,酶解时间2.0h,酶解温度61℃,酶解pH6.1时,香椿叶TDF提取率最高可达74.01%;所得TDF杂质含量低,生理活性好;持水力和膨胀力分别为6.50g/g和4.50mL/g。

Abstract: The method of single factor test and response surface analysis was adopted to optimize the enzymatic extraction of total dietary fiber ( TDF) from leaves of Toona sinensis with cellulose and amylase ( 1∶1) .The results showed that enzyme dosage 0.5%, enzymolysis time 2.0h, temperature 61℃, and pH6.1. The TDF extracting rate was 74.01% under these optimum conditions with low levels of impurities.The physiological activation was good, the holding water capacity and swelling property were 6.50 g /g and 4.50 mL /g respectively.