缫丝蚕蛹蛋白酶法水解条件优化

Optimization of protease hydrolysis conditions on silkworm pupa protein

摘要: 目的:利用酶水解缫丝蚕蛹蛋白,拟为其深度开发利用提供参考。方法:利用蛋白酶水解缫丝蚕蛹蛋白,并用分光光度法测定各指标。结果表明:单酶水解时,实验所用的8种酶中以蚕蛹蛋白水解专用酶的水解度最高,最佳水解条件是底物浓度7%,温度53℃,pH7.2,加酶量3%,水解度达到21.44%。

Abstract: Purpose: Enzyme hydrolysis of silkworm pupa protein was studied in order to provide references to deeply exploit silkworm pupa protein.Methods: Protease was used to hydrolyze silkworm pupa protein and spectrophotometry was used to detect indicators.Results: When hydrolyzed by single protease, dedicated protease for silkworm pupa protein showed the highest hydrolysis degree out of 8 proteases used in the experiment, the best hydrolysis conditions were: the substrate concentration was 7%, 53℃, pH7.2, the additive amount of protease was 3%, and the hydrolysis degree turned out to be 21.44%.