响应面法优化去皮绿豆多糖提取工艺技术的研究
Study of extracting water-soluble polysaccharide from dehulled mung bean by response surface methodology
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摘要: 以去皮绿豆为主要原料,通过单因素实验,探讨了提取温度、提取时间、料液比、提取次数等因素对绿豆多糖得率的影响。并且在单因素结果的基础上,利用响应面分析法优化提取绿豆多糖的最佳工艺条件。实验结果表明,水提醇沉法提取去皮绿豆多糖的最佳工艺参数为:提取温度95.5℃,提取时间3h,料液比为21∶1,提取1次,在此条件下去皮绿豆中可溶性粗多糖的得率可以达到4.32%。经Sevag法脱蛋白后,其中多糖纯度为50.35%,蛋白含量为31.26%。Abstract: Experiments concerning extracting polysaccharide from dehulled mung bean have been conducted.Particularly, the effects of factors ( extraction time, the ration of raw materials to water, extraction times and extraction temperature) on the yield of polysaccharide had been studied as well. Based on the single- factor experiments, response surface methodology had been employed to optimize extraction conditions. Results indicated that the optimum conditions for polysaccharide from dehulled mung bean extraction were: water temperature of 95.5℃, a ratio of water to raw materials of 21 ∶ 1 ( V/m) and an extraction time of 3h. Under this conditions, the yield of polysaccharide reached 4.32%. Following this, Sevag methodology had been used to remove the protein contained in polysaccharide as well.After deproteination, the content of polsaccharide reached50.35% while there was still approximately 31.26% of protein.