Abstract: A method for the determination of urotropine in dried beancurd sticks was established by UPLC-MS/MS.The sample was extracted with acetonitrile as extraction solvents, then further purified by PXA solid phase extraction column.The analyte was determined by multi- reaction monitoring ( MRM) mode was employed for the quantitative determination, and quantified by the external standard curve.The separation was performed on a UPLC ACQUITY BEH HILIC column by gradient elution with a system of water ( containing 10 mmol /L ammonium acetate buffer) -acetonitrile as mobile phase.Results showed that the calibration curve had good linearity.The developed method exhibited good linearity over the range from 1 to 20μg /L.The minimum detection limit was 1μg /L. The average recovery rate for urotropine was 81.67% ~ 101.67%.The method is simple, accurate and suitable for the identification and quantification.