Abstract: Ginsenosides was one of the main active ingredients in ginseng.It has many pharmacological effects that include immunity enhancement, antioxidant, anti- fatigue, antitumor effect and so on.Nowadays, how to obtain the high quality of ginsenosides had became a hot research topic.Therefore, this review summarized the method of the extraction and separation technology of ginsenosides and made a comparison on it to confirm the best method of the extraction and separation of ginsenosides so that it provided some scientific basis on the development and application of ginsenosides.