松木层孔菌多糖PP60-S1分离、结构表征及抗氧化活性研究

Study on isolation, structural characterization and antioxidant activity evaluation of polysaccharide PP60-S1 from Phellinus pini

摘要: 松木层孔菌子实体水提物经乙醇分级醇沉和DEAE-Sepharose Fast Flow离子柱层析及Sephacryl S-300凝胶柱层析纯化,获得均一多糖PP60-S1,其分子量为3.56×104u,是由葡萄糖、甘露糖和半乳糖组成的杂多糖,单糖摩尔比为9.24∶1.00∶0.76,甲基化分析PP60-S1主要由1-Glc、1,3-Glc、1,6-Glc、1,3-Gal、1,6-Gal、1,3,6-Man 6种糖苷键连接方式,主链主要由1,6-Glc构成。PP60-S1的体外抗氧化活性研究表明其具有一定的DPPH自由基和ABTS+自由基清除活性以及FRAP总抗氧化能力。

Abstract: The homogeneous polysaccharide PP60-S1 was obtained by water extraction, ethanol precipitation and purified by DEAE-Sepharose F. F. and Sephacryl S-300 from the fruiting bodies of Phellinus pini. The polysaccharide PP60-S1 was composed of glucose, mannose and galactose with a molar ratio of 9.24∶1.00∶0.76, with an average molecular weight of 3.56×104u. The results of methylation analysis indicated that six kinds of glycosidic linkages were detected in the structure of PP60-S1:1-Glc、1, 3-Glc、1, 6-Glc、1, 3-Gal、1, 6-Gal、1, 3, 6-Man, respectively. The main chain contained mainly 1, 6-Glc. Otherwise, the polysaccharide PP60-S1 exhibited a certain degree of antioxidant activity, evaluated by in vitro assay of DPPH·, ABTS+· and ferricreducing antioxidant power (FRAP) .