Abstract: In order to optimize the ultrasonic assisted extraction of alkaloid in Zanthoxylum bungeanum, the single factor and response surface design were applied. The antioxidant was evaluated by 2, 2-Diphenyl-1-picrylhydrazyl (DPPH) radical scavenging, and tyrosinase inhibition activity was conducted by the mushroom tyrosinase inhibition. The results showed that the optimum extracting conditions of Zanthoxylum bungeanum alkaloid were as follows:ethanol concentration 79%, solid-liquid ratio 1∶16g/mL, ultrasonic time 11 min, ultrasonic power 233 W. The IC50 value of the DPPH radical scavenging of Zanthoxylum bungeanum alkaloid was0.0599mg/mL, and the IC50 value of tyrosinase inhibition was 1.212mg/mL. These indicated that Zanthoxylum bungeanum alkaloid exhibited some antioxidant and tyrosinase inhibition activities, but the activities were both lower than VC.