紫甘蓝花色苷组成及抗猪传染性胃肠炎病毒活性分析
Analysis of the anthocyanin profiles and anti- TGEV activity from purple cabbage
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摘要: 为在体外探寻紫甘蓝花色苷(Anthocyanins from Purple Cabbage,APC)抗猪传染性胃肠炎病毒(Transmissible gastroenteritis virus of swine,TGEV)的活性效果。本研究以APC粉为原料,采用高效液相色谱仪与质谱仪联机的方法鉴定其花色苷成分后,在体外将APC作用于感染TGEV的猪睾丸细胞系(swine testicle,ST)单层表面,通过研究APC对TGEV诱导ST细胞的形态学变化和细胞存活情况,评价APC是否具有抗TGEV的活性作用。结果表明:紫甘蓝花色苷粉中至少含13种花色苷成分,其中主要为矢车菊色素的糖苷;APC对细胞无毒性的最大安全质量浓度为100μg/m L;APC在安全浓度范围内能够显著抑制TGEV诱导的ST细胞形态变化,降低凋亡。因此,紫甘蓝花色苷具有抗TGEV的生物活性作用。Abstract: To explore the inhibitory action of anthocyanins from purple cabbage( APC) for transmissible gastroenteritis virus of swine( TGEV) in vitro. APC powder as raw material,the sample of anthocyanins structure were identified by HPLC- MS method,APC were acted on disseminated TGEV in monolayer ST cells,then its influence on the morphological changes as well as cell survival were researched,it was evaluated whether APC had the active role of anti- TGEV. The results showed that the purple cabbage contained at least 13 kinds of anthocyanins,the main component was cyaniding- glucosides.The maximum safe mass concentration of APC was100μg / m L for ST cells nontoxicity.Introduced TGEV to ST,APC could restrain ST cell morphological changes and less death rate under the 100μg / m L. In summary,anthocyanins from purple cabbage had significantly anti- TGEV biological activity.
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