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中国精品科技期刊2020
韦朝阳, 贺亮, 李卫旗, 邵双双, 冯依力, 程俊文. 不同中药水提物对蝉拟青霉液体深层发酵和产物活性的影响[J]. 食品工业科技, 2015, (03): 168-171. DOI: 10.13386/j.issn1002-0306.2015.03.026
引用本文: 韦朝阳, 贺亮, 李卫旗, 邵双双, 冯依力, 程俊文. 不同中药水提物对蝉拟青霉液体深层发酵和产物活性的影响[J]. 食品工业科技, 2015, (03): 168-171. DOI: 10.13386/j.issn1002-0306.2015.03.026
WEI Chao- yang, HE Liang, LI Wei-qi, SHAO Shuang-shuang, FENG Yi-li, CHENG Jun-wen. Effects of different kinds of aqueous herbal extracts on submerged fermentation and product activity of Paecilomyces cicadae[J]. Science and Technology of Food Industry, 2015, (03): 168-171. DOI: 10.13386/j.issn1002-0306.2015.03.026
Citation: WEI Chao- yang, HE Liang, LI Wei-qi, SHAO Shuang-shuang, FENG Yi-li, CHENG Jun-wen. Effects of different kinds of aqueous herbal extracts on submerged fermentation and product activity of Paecilomyces cicadae[J]. Science and Technology of Food Industry, 2015, (03): 168-171. DOI: 10.13386/j.issn1002-0306.2015.03.026

不同中药水提物对蝉拟青霉液体深层发酵和产物活性的影响

Effects of different kinds of aqueous herbal extracts on submerged fermentation and product activity of Paecilomyces cicadae

  • 摘要: 通过分别向培养基中添加不同的中药水提物进行蝉拟青霉液体发酵培养,探究其对蝉拟青霉发酵过程中生物量、胞外和胞内多糖的产量及其产物活性的影响。结果表明,黄芪、薏苡仁、枸杞的水提物分别对蝉拟青霉生物量、胞外和胞内多糖的合成促进效果最为显著(p<0.05),分别较对照实验提高了135.82%、48.90%、137.18%;而金银花和山楂水提物不利于蝉拟青霉的发酵培养;对于产物活性,结果显示枸杞水提物能同时显著(p<0.05)提高蝉拟青霉胞外和胞内多糖对DPPH自由基的清除能力,分别为对照实验的1.17倍和1.38倍。综合比较上述结果,枸杞水提物是蝉拟青霉液体发酵的最适中药成分,其最佳添加量为10g/L。 

     

    Abstract: The effects of different aqueous extracts of herbs on biomass,the yield of exopolysaccharide and intracellular polysaccharide as well as its antioxidant activities were studied by the means of adding these water extracts to culture medium of P. cicadae in submerged fermentation process. Results showed that the positive effects of aqueous extracts of Astragalus membranaceus,Coix lachryma- jobi,Lycium barbarum on biomass,the synthesis of exopolysaccharide and intracellular polysaccharide separately were the most remarkable ones( p < 0.05),which increased by 135.82%,48.90%,137.18% compared to the control,respectively.While the aqueous extracts of Flos Lonicerae and Crataegus pinnatifida were not beneficial to fermentation of P. cicadae. It was indicated that the activities of metabolites were improved significantly by the aqueous extract of Lycium barbarum,the scavenging ability of DPPH radical of exopolysaccharide and intracellular polysaccharide were 1.17 folds and1.38 folds of those of the control,respectively.Synthesizes these results,aqueous extract of Lycium barbarum was selected as the optimum herb ingredient for submerged fermentation of P.cicadae,and its proposed dosage was10 g / L.

     

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