Abstract: A method using dispersive solid phase extraction( DSPE) cleanup followed by high performance liquid chromatography- mass spectrometry( HPLC- MS) has been established for determination of acetamiprid in soil and cabbage.In this method,the clean- up was carried out using Mg SO4,primary secondary amine( PSA) and C18with1% acetic acid- acetonitrile as extract solvent.0.1% formic acid in water and methanol were used as mobile phase,C18 chromatographic column was used to separation in determinating samples on HPLC- MS.In the range of 0.01~2mg / L,the good linear relationship was showed between concentration and peak area,R2= 0.9994,the LOQ of this method was 0.01 mg / kg,which was below the MRL( 0.5mg / m L). At the spiked levels of 0.05,0.5,1.0mg / kg,the recoveries of acetamiprid were 84.8% ~ 107% in cabbage and 96.5% ~ 115% in soil,and the relative standard deviation( RSD) was less than 10.92%. This method can meet the requirements of the limited determination of acetamiprid residues in cabbage and soil.