Abstract: The systematic observation,statistics,test on chilling injury symptoms,chilling injury index,chilling injury incidence,membrane permeability,hardness,soluble solids content,titratable acid and vitamin C of postharvest86-1 Hami melon( Cucumis melo L.) fruit under storage temperature 1,3,5℃ were made in order to study the influence on chilling injury and quality of postharvest 86- 1 Hami melon( Cucumis melo L.) fruit under different storage temperatures. The results showed that the occurrence of chilling injury of postharvest 86- 1Hami melon( Cucumis melo L.) was connected with storage temperature and duration time,under 1℃ the incidence of chilling injury was the earlier,the chilling injury index,chilling injury incidence and membrane permeability of postharvest 86-1 Hami melon( Cucumis melo L.) fruit were higher; hardness,soluble solids content,and vitamin C declined rapidly,titratable acid was increased at first and decreased subsequentl. Fruit quality of postharvest 86- 1 Hami melon( Cucumis melo L.) fruit was maintained better under 3℃. There was no incidence of chilling injury,but the quality of postharvest 86-1Hami melon( Cucumis melo L.) fruit was declined quickly at later,and decay was serious under 5℃.