Abstract: In the present study,response surface methodology was employed to optimize the ultrasound-assisted hot water extraction conditions of polysaccharides from tea flower. Based on the results of single- factor experiments,the optimal conditions for polysaccharides extraction were determined by Central Composition Design as follows:ultrasonic time 12 min,ultrasound power 540 W,ratio of solvent to material 30 ∶1m L/g. Under such conditions,the extraction yield of polysaccharides was 7.69%. The results suggested that the regression models were accurate and adequate for the extraction of tea polysaccharides from tea flower.