Abstract: Intestinal microflora was considered as one of the major sources to cause the corruption of aquatic products. As a new- type environmental disinfectant, acidic electrolyzed water( AEW) had gradually been applied to control the security of aquatic products,which was receiving more and more attentions due to its efficient broad-spectrum sterilization. In this paper,PCR-DGGE technology was used to analyze the variation of intestinal microflora diversity in Penaeus vannamei stored at 0℃,4℃ and 25℃,which were treated by tapwater( TW) and AEW, respectively. Compared with TW group, samples under AEW showed a decreasing diversity of intestinal microflora during storage,which indicated that intestinal microflora diversity was significantly affected by AEW. The results would be used to provide a theoretical reference for the security of aquatic products.