Abstract: With the methods of morphology,physiology and biochemistry,26 S r DNA sequence homology analysis and phylogenetic tree,strains from Chongqing Yunyang loquat flower and rape flower bee bread were identified.The results showed that: yeast strains separated from the loquat flower bee bread had high homogeneity with Zygosaccharomyces rouxii strain( KC146358.1),which were determined to be Zygosaccharomyces rouxii. Through morphological and physiological identification,three of the five strains separated from the bee bread of loquat flower were identified as Lactococcus lactis subsp.1actis,which were named MRS2,MC1,and LB1 and the other 2strains were Enterococcus durans named MRS1 and LB2.In the same way,four of the seven strains separated from the bee bread of rape flower were identified as Lactobacillus plantarum,which named MRS3,MC2,LB3 and LB4.The other three strains were Lactobacillus rhamnosus named MRS4,MRS5 and LB5.The separation of these strains of provided scientific content for the research of bee bread microbial in Yunyang area and provides a choice of strains for the development of bee bread products.