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中国精品科技期刊2020
李海杰, 葛永红, 董柏余, 龚迪, 刘文妮, 毕阳. 三种贮藏低温对厚皮甜瓜果实活性氧产生和清除的比较[J]. 食品工业科技, 2015, (05): 325-328. DOI: 10.13386/j.issn1002-0306.2015.05.060
引用本文: 李海杰, 葛永红, 董柏余, 龚迪, 刘文妮, 毕阳. 三种贮藏低温对厚皮甜瓜果实活性氧产生和清除的比较[J]. 食品工业科技, 2015, (05): 325-328. DOI: 10.13386/j.issn1002-0306.2015.05.060
LI Hai-jie, GE Yong-hong, DONG Bo-yu, GONG Di, LIU Wen-ni, BI Yang. A comparison of reactive oxygen species production and scavenging in muskmelon fruits during storage under three low temperatures[J]. Science and Technology of Food Industry, 2015, (05): 325-328. DOI: 10.13386/j.issn1002-0306.2015.05.060
Citation: LI Hai-jie, GE Yong-hong, DONG Bo-yu, GONG Di, LIU Wen-ni, BI Yang. A comparison of reactive oxygen species production and scavenging in muskmelon fruits during storage under three low temperatures[J]. Science and Technology of Food Industry, 2015, (05): 325-328. DOI: 10.13386/j.issn1002-0306.2015.05.060

三种贮藏低温对厚皮甜瓜果实活性氧产生和清除的比较

A comparison of reactive oxygen species production and scavenging in muskmelon fruits during storage under three low temperatures

  • 摘要: 本文以厚皮甜瓜‘金红宝’为试材,研究了三种贮藏低温(3、7和9℃)对果实O-2·产生速率和H2O2含量,超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽还原酶(GR)和抗坏血酸过氧化物酶(APX)活性的影响。结果表明,7℃和9℃下果实O-2·产生速率在前期(第4d)显著增加,而3℃下在后期(第12d)逐渐积累;3℃下果实的H2O2含量前期明显升高,而7℃和9℃下在中期(第8d)和后期逐渐升高。贮藏前期7℃下果实SOD、GR和APX的活性明显高于3℃和9℃处理,而3℃下果实的CAT活性最低。上述结果显示,3℃下大量的H2O2积累以及较低的抗氧化酶活性是导致甜瓜果实冷害发生的重要原因,7℃下果实维持了良好的活性氧产生和清除平衡,为厚皮甜瓜贮藏的适宜温度。 

     

    Abstract: Muskmelon fruit( cv.Jinhongbao) was used as material to study the effect of three low storage temperatures( 3,7,9℃) on the production rate of superoxide anion( O-2·),accumulation of hydrogen peroxide( H2O2) and the activities of superoxide dismutase( SOD),catalase( CAT),glutathione reductase( GR) and ascorbate peroxidase( APX) during storage.The results showed that the production rate of O-2·was increased at the early stage( 4d) of storage at 7℃ and 9℃,but significantly enhanced at the late stage( 12d) of storage at 3℃.The accumulation of H2O2 was significantly increased at the early stage of storage at 3℃.However,it was gradually increased in the mid( 8d) and late( 12d) stage of storage at 7℃ and 9℃.At the early stage of storage,the activities of SOD,GR and APX in muskmelons stored at 7℃ were higher than that stored at 3℃ and 9℃,and the activity of CAT in fruit was the lowest at 3℃.These results indicated that a large accumulation of H2O2 and the lower activities of the antioxidant enzymes in fruit stored at 3℃ may lead to chilling injury of muskmelons. The fruit stored at 7℃keep the balance of reactive oxygen species production and scavenging,indicating 7℃ is suitable temperature for storage of muskmelons.

     

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