凝胶色谱-气相色谱法测定11种动植物油脂中角鲨烯的含量

Determination of squalene in edible oils of plant and animal by gel permeation chromatography-gas chromatography method

摘要: 动植物食用油经环己烷-乙酸乙酯（1∶1,V/V）萃取后,用凝胶色谱法对油样品进行净化处理,去除样品中大分子的油脂,再采用气相色谱法对动植物食用油中角鲨烯进行测定,以建立多种动植物油中角鲨烯的凝胶色谱-气相色谱（GPC-GC-FID）测定方法。在一定的实验条件下,角鲨烯在9.40⁹40μg·m L-1范围内呈现良好线性关系,相关系数R2为0.9999,方法回收率为95.7%,RSD为3.0%。该方法处理后的食用油样品中干扰基质少,灵敏度高,可有效避免检测过程中仪器的污染,广泛应用于动植物食用油中角鲨烯的检测,为评判动植物食用油标准提供依据。

Abstract: To established the gel chromatography-gas chromatography(GPC-GC-FID) method for the assay of squalene in several edible oils of plant and animal,the oils were extracted by cyclohexane-ethyl acetate(1∶1,V / V), then purified by GPC to remove macromolecule grease, and then squalene was determined by gas chromatography. Under the experimental condition,squalene showed a good linear relationship in the range of9.40~940μg·m L-1,R2 was 0.9999. The recovery was 95.7%,RSD was 3.0%. The method had the advantage in small sample matrix interference,high sensitivity,avoiding contamination instrument,could be widely used in the analysis of squalene in edible oils of plant and animal sample,which provided the basis for judging criteria of edible oils of plant and animal sample.