蓬莪术多糖体外抗氧化活性研究

Antioxidant activities of polysaccharides from rhizomes of the herb Curcuma phaeocaulis in vitro

摘要: 以多年生草本药用植物蓬莪术根茎为原材料,热水浸提法提取多糖,测定蓬莪术多糖的总糖含量、糖醛酸含量和蛋白质含量,并对蓬莪术多糖结构进行红外光谱分析。通过体外抗氧化实验研究蓬莪术多糖对自由基的清除能力以及总还原能力。结果表明,蓬莪术多糖的总糖质量分数为56.13%,糖醛酸质量分数为8.65%,蛋白质质量分数为4.53%。体外抗氧化活性研究结果表明对DPPH自由基、羟基自由基和超氧阴离子有明显清除作用和较强的还原能力,其清除自由基的IC50值分别为0.29、0.44、0.36mg/m L。

Abstract: Water-soluble polysaccharides were obtained from rhizomes on roots of the herb Curcuma phaeocaulis Valeton. In addition,the total contents of carbohydrate,uronic acid and protein of C. phaeocaulis polysaccharide were measured by chemical methods,and the structural characteristic of CPP was detected by FT-IR spectra.Furthermore,the antioxidant activities of CPP were evaluated on the basis of DPPH,hydroxyl radical,superoxide anion radicals scavenging ability and reducing power in vitro. The results showed that the contents of carbohydrate,uronic acid and protein were 56.13%,8.65% and 4.53%,respectively. The results showed that the CPP possessing remarkable free radical scavenging activity and a good reducing power in vitro antioxidant assay. The IC50 values of scavenging free radicals were 0.29,0.44,0.36mg/m L,respectively.