响应面设计优化复合酶法提取菊苣菊粉工艺研究
Study on optimization on composite enzyme extraction process of inulin from Cichorium intybus L. by response surface design
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摘要: 以菊苣根为原料,采用果胶酶和纤维素酶复合法提取菊粉。在单因素的基础上,选取料液比、温度、时间、p H为自变量,以菊粉提取率为响应值,通过Box-Behnken响应面实验设计对其提取工艺参数进行优化。结果显示复合酶法提取菊苣菊粉最优工艺为:料液比1∶8g/m L,提取温度60℃,提取时间2.5h,提取p H6.0,复合酶配比为1∶2的条件下,菊粉提取率可达到54.63%。与热水浸提法相比,提取率高出64.10%。Abstract: In this experiment,with chicory root as a raw material,pectinase and cellulase compound preparations was used to extract inulin. Four extraction parameters including the ratio of material and solvent,extraction temperature,extraction time and extraction p H were optimized as the result of the conditions for maximum extraction of inulin from Cichorium intybus L. by Box-Behnken response surface design methodology based on the inulin extraction rate as a response value. Results showed that the optimum condition of inulin extraction was as follows the ratio of material and solvent 1 ∶8g/m L,extraction temperature 60℃,extraction time 2.5h,extraction p H6.0,and the ratio of compound enzymes 1∶2. Under this condition inulin extraction rate was up to54.63%. The extraction rate increased by 64.10%,compared with the method of hot water extraction.