响应面优化水提取燕麦抗真菌蛋白的研究

Study on the optimization of water extraction of oat antifungal protein by response surface anaylsis

摘要: 以裸燕麦籽粒为原料,利用水提取燕麦蛋白中的抗真菌蛋白(antifungal protein),用打孔法观察并测量抑菌圈直径,在单因素实验的基础上,应用响应面分析法,探讨提取时间、提取温度、p H以及液料比对燕麦抗真菌蛋白抑菌性的影响,确定燕麦抗真菌蛋白的最佳水提工艺。实验结果:当提取时间12h,提取温度30℃,p H7.5,液料比12∶1m L/g时,抑菌圈直径达到最大为18.10mm。

Abstract: Antifungal proteins were extracted from seeds of naked oat by water extraction method. The antifungal activity of the protein was evaluated by hole method. Based on single factor method,response surface analysis were used to explore the effects of extraction time,extraction temperature,p H and liquid-to-solid ratio on antifungal activity of oat antifungal protein. These methods determined the optimal water extraction conditions of oat antifungal protein. Results:The optimal extraction time,extraction temperature,p H and liquid-to-solid ratio were 12 h,30℃,7.5,12∶1m L/g. The actual maximum diameter of inhibition zone was 18.10 mm.