Box-Behnken设计-效应面法优化连钱草多糖提取工艺

Optimization of extraction process of polysaccharides from Glechoma longituba by Box-Behnken design-response surface method

摘要: 对连钱草多糖进行提取,并通过单因素及响应面实验对提取的四个因素:p H、液固比、提取温度和提取时间进行了优化。结果表明:当提取工艺为p H7.2、液固比33∶1(m L/g)、提取温度89℃、提取时间160min时,多糖提取率最高,可达3.40%±0.05%,与理论值3.39%接近。此研究为连钱草多糖的进一步开发和利用提供了理论依据。

Abstract: Polysaccharides from Glechoma longituba were extracted in the present study,and four factors including p H,liquid-solid ratio,extraction temperature and time were optimized by univariate tests and response surface methodology. The results showed that the highest yield of polysaccharides extracted from Glechoma longituba reached 3.40% ±0.05% when the process was p H7.2,liquid-solid ratio 33 ∶1(m L/g),temperature 89℃ and extraction time 160 min. This research might provide a theoretical basis for the further development and utilization.