Abstract: In order to determine the effect of two exogenous signal molecules- jasmonic acid methyl ester(Me JA) and ethephon on enzymatic browning and membrane lipid peroxidation reaction for fresh-cut fruit and vegetables. This experiment taked fresh-cut Fuji apple as material,after treated with 10μmol/L Me JA and 10μL/L ethephon,analysis the change of enzymatic browning and enzymes membrane lipid peroxidation related. The results showed that Me JA and ethephon treatments could effectively inhibit LOX activity(p<0.05),and controlled the increase of relative electrical conductivity and the content of MDA,and color loss reduction. Ethephon treatments could effectively inhibit CAT activity,and slow down the decline rate of SOD activity,but the effect of CAT and SOD activity for Me JA treatments was not obvious. Me JA and ethylene treatments launched the defense reaction of the fresh-cut Fuji apple,reduced the rate of enzymatic browning and caused the activities of the key enzymes related with defense responses increased,with prolong the storage period and maintain the fresh quality(p<0.05).