响应曲面法优化糙米富硒发芽工艺研究

Optimization of germination of selenium enrichment of brow rice germination by response surface method

摘要: 为优化糙米富硒发芽工艺,以糙米发芽率及有机硒含量为指标,通过单因素和Box-Behnken组合设计,研究了亚硒酸钠质量浓度、培养温度、培养时间及其交互作用对糙米发芽和富集硒的影响,优化后的工艺参数为:亚硒酸钠质量浓度68 mg/L、培养温度30℃、培养时间31 h。在此条件下糙米发芽率达到81.9%,有机硒含量达到0.870 mg/kg。

Abstract: To obtain both optimal germination rate and organic- selenium content,germination conditions such as sodium- selenite concentration in soaking solution,cultivation time and cultivation temperature were investigated by Box- Behnken central composite design( CCD) combined with response surface methodology.The results showed that the optimal parameters were as follows: sodium selenite solution 68 mg / L,cultivation temperature 30 ℃,cultivation time 31 h. Under these conditions,germination rate reached 81.9% and the contents of Se reached0.870 mg / kg.