Abstract: The protective effect of peppermint polyphenols on CCl4-induced liver damage in mice was studied in this paper. The results showed that the high dosage treatment(400 mg/kg BW·d) by gavage of peppermint polyphenols significantly inhibited the increase of AKP,ALT,AST activity(p <0.05). The pathological tissue biopsies showed that liver cells of mice in high dose group remained intact with a little spotty necrosis,and had no large necrosis area,it further indicated the peppermint polyphenols had protective effect on liver damaged by CCl4. On the other,the high dosage treatment by gavage of peppermint polyphenols significantly inhibited the elevation of content of TC and TG in serum,and increased the SOD,CAT and T-AOC level in liver,and deceased the content of MDA in liver(p<0.05). It showed that peppermint polyphenols had potential to be developed in the aspect of preventing and improving liver damage.