植物源性转基因食品PCR衍生技术研究进展

Advance in PCR-derived technologies for plant-derived genetically modified food

摘要: 转基因食品尤以植物源性转基因食品的安全性已成为公众关注的焦点。针对植物源性转基因食品甄别与安全性评估,已有多种检测手段得到广泛应用。PCR衍生技术因为具有高特异性、高敏感性等技术优势,为植物源性转基因食品的快速、准确检测提供了有效方法。PCR衍生技术包括多重PCR技术、实时荧光定量PCR技术、多重实时荧光定量PCR技术、多重巢式PCR技术以及多重PCR-DHPLC技术。本文就植物源性转基因食品检测的PCR衍生技术的研究进展作简要综述。

Abstract: Safety of genetically modified food especially plant-derived genetically modified food are increasingly becoming attentive focus of the public. Many detection methods have been developed for the detection of discrimination and safe evaluation of plant-derived genetically modified food. PCR-derived technologies are sensitive and specific and so have provided rapid,accurate detection methods for plant-derived genetically modified food. PCR-derived technologies include multiplex PCR,real-time PCR,multiplex real-time PCR,multiplex nested PCR, multiple PCR- DHPLC. In this review, these PCR- derived methods should be summarized in brief.